Alpha Tubulin Molecular Weight Kda

Use a concentration of 1 µg ml.

Alpha tubulin molecular weight kda. α tubulin also called tubulin α 4a tuba4a is mapped to human chromosome 2q35. Tubulin is the major constituent of microtubules. Can be blocked with alpha tubulin peptide ab23537. Belongs to the.

The β tubulin subunit is exposed on the plus end of the microtubule while the α tubulin subunit is exposed on the minus end. Use a concentration of 0 5 µg ml. Detects a band of approximately 55 kda predicted molecular weight. Use a concentration of 1 µg ml.

Volumes are for 10 cm x 10 cm. The gene codes for a member of the α tubulin family and contains 448 amino acids. It binds two moles of gtp one at an exchangeable site on the beta chain and one at a non exchangeable site on the alpha chain. Electrotransfer to nitrocellulose membrane.

Tuba4a belongs to the subfamily of tubulin which is the major building block of microtubules. The α subunit of tubulin has molecular weight of 50 000. Loading of prestained molecular weight markers 13953 5 µl lane to verify electrotransfer and biotinylated protein ladder 7727 10 µl lane to determine molecular weights are recommended. 50 kda and share considerable homology.

In addition to α and β tubulin several other tubulins have been identified bringing the number of distinct tubulin classes to seven. Tubulin is a heterodimer that consists of α tubulin and β tubulin. Both subunits have a molecular weight of approx. Membrane blocking and antibody incubations.

50 kda can be blocked with human beta tubulin peptide ab20775. Prestained protein marker broad range 11 190 kda. Detects a band of approximately 50 kda predicted molecular weight. Perform heat mediated antigen retrieval before commencing with ihc staining protocol.

Use at an assay dependent concentration. Detects a band of approximately 51 kda predicted molecular weight. Each has a molecular weight of approximately 50 kda. Abcam recommends using milk as the blocking agent.

Detects a band of approximately 50 kda predicted molecular weight. To form microtubules the dimers of α and β tubulin bind to gtp and assemble onto the ends of microtubules while in the gtp bound state. Pa5 19489 detects alpha tubulin which has a predicted molecular weight of approximately 50 kda.