Alpha Tubulin Molecular Weight Santa Cruz

Paraffin embedded endometrium tissue of a 33 year old female was incubated with ab4074 1 25 dilution for 30 mins at room temperature.

Alpha tubulin molecular weight santa cruz. Sc 8035 santa cruz biotechnology inc. Pa5 19489 detects alpha tubulin which has a predicted molecular weight of approximately 50 kda. Target information alpha tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins which are each about 55 kda mw are homologous but not identical.

Alpha beta tubulin heterodimer is the basic building block of microtubules and this intracellular. Sc 5286 santa cruz biotechnology inc. Santa cruz biotechnology inc. α tubulin antibody tu 02 is a mouse monoclonal igm kappa light chain provided at 200 µg ml.

The following lists a number of tubulin antibodies from various sources. Alpha tubulin belongs to a large superfamily of tubulin proteins. Tubulin antibody sigma monoclonal anti α tubulin antibody produced in mouse. Santa cruz biotechnology inc.

Antigen retrieval was performed by heat induction in citrate buffer ph 6. Alpha tubulin antibody sigma anti tubulin sigma tubulin antibody sigma. Anti alpha tubulin antibody b 7 is recommended for detection of α tubulin of mouse rat and human origin by wb ip if ihc p fcm and elisa. Radioimmunoassay indirect immunofluorescence cultured human or chicken fibroblasts 1 2 000 western blot human fibroblast cell extract 1 4 000.

After electrophoresis the blot was stained with bodipy tr x succinimidyl ester red signal to detect total protein. These maintain cell structure provide platforms for intracellular transport and are also involved in cell division. Ab4074 was tested in a tissue microarray tma containing a wide range of normal and cancer tissues as well as a. Anti alpha tubulin antibody tu 02 is recommended for detection of α tubulin of mouse rat human and porcine origin by wb ip if ihc p fcm and elisa.

Also reactive with additional species including and canine bovine and porcine. α and β tubulins form heterodimers which multimerize to form a microtubule. Anti tubulin antibody products from santa cruz biotechnology inc. Ab4074 staining alpha tubulin in the glandular cells of the endometrium.

Samples of protein molecular weight standards product p 6649 containing decreasing amounts of a tubulin were run on an sds polyacrylamide gel and blotted onto a pvdf membrane. Raised against amino acids 1 451 representing full length α tubulin of porcine origin. α tubulin antibody b 7 is a mouse monoclonal igg 2a kappa light chain provided at 200 µg ml. There are a number of different subtypes that have a molecular weight of 50kda and are able to bind to beta tubulin forming a heterodimer that polymerizes to microtubules as part of the cytoskeleton.

After staining the blot was incubated with a mouse monoclonal anti a tubulin antibody.