Alpha Tubulin Molecular Weight Western Blot

Anti alpha tubulin antibody dm1a loading control ab7291 at 1 2500 dilution. A431 lysate lysates proteins at 20 µg per lane. I observed a very strong band using anti tubulin antibody around 55kd molecular weight.

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Cst A B Tubulin Antibody

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Cst A Tubulin Antibody

Western blot anti alpha tubulin antibody dm1a loading control ab7291 this image is courtesy of an abreview submitted by elena kashuba.

Alpha tubulin molecular weight western blot. The α subunit of tubulin has molecular weight of 50 000. Western blot anti alpha tubulin antibody microtubule marker ab18251 all lanes. α tubulin also called tubulin α 4a tuba4a is mapped to human chromosome 2q35. α tubulin also called tubulin α 4a tuba4a is mapped to human chromosome 2q35.

α subunit of tubulin has molecular weight of 50 000. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Tyrosine ligase adds a c terminal tyrosine to monomeric alpha tubulin. The gene codes for a member of the α tubulin family and contains 448 amino acids.

Anti alpha tubulin antibody microtubule marker ab18251 at 0 5 µg ml lane 1. 30 ugs of protein samples were used for this western blot to detect tubulin in pc3 cell lysates. Western blot analysis of alpha tubulin was performed by loading 20 µg of a431 lane1 raji lane2 mda mb 231 lane3 hct 116 lane4 hela lane5 du 145 lane6 pc 3 lane7 and caco 2 lane8 cell lysate using novex nupage 12 bis tris gel product np0341box xcell surelock electrophoresis system product ei0002 novex sharp pre stained protein standard lc5800 and iblot. Tuba4a belongs to the subfamily of tubulin which is the major building block of microtubules.

α subunit of tubulin has molecular weight of 50 000. Alpha tubulin antibody dm1a nb100 690 analysis of hela and cos 7 lysates. The gene codes for a member of the α tubulin family and contains 448 amino acids. Hela lysate lane 2.

50 kda additional. 50 kda observed band size. Alpha tubulin molecular weight. Secondary all lanes.

Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter with a theoretical molecular weight of 50 kda. Tuba4a belongs to the subfamily of tubulin which is the major building block of microtubules. Western blotting is an important technique used in cell and molecular biology. Alexa flour goat polyclonal to rabbit igg 700 at 1 10000 dilution predicted band size.

By using a western blot researchers are able to identify specific proteins from a complex mixture of proteins.